Macro- and micro-nutrient–based multiplex stress conditions modulate in vitro tumorigenesis and aggressive behavior of breast cancer spheroids

The aggressive nature of a tumor is presumably its inherent one, but different environmental cues can manipulate it in many ways. In this context, the influence metabolic stresses on behavior needs to be analyzed understand their far-reaching implications aggression and dormancy. This work investigates facets tumor, such as tumorigenic capacity, phenotype, migration, under multiple stress conditions. Non-invasive invasive multicellular spheroids (MTS) were created subjected conditions, namely glucose, amino acid, oxygen deprivation. Altered MTS has been evaluated context vitro tumorigenesis, spheroid formation mRNA profile, recruitment mesenchymal stem cells. conditions observed negatively impact tumorigenesis process non-invasive breast cancer While seemingly influences growth phenotype spheroids, does not alter organization sub-cellular entities significantly. Metabolic transcriptomic landscape hypoxic, angiogenic, ECM deformation, glycolysis shift, protein starvation-related gene clusters. MTSs do adhere or migrate stress, they exhibit modalities migration when rescued. Invasive after rescue, increased aggressiveness. Furthermore, stressed was control Multiplex could while influencing physiology along with pattern tumor-stromal crosstalk.